The EJC is deposited on mRNA during splicing. RIPiT-Seq allows researchers to purify endogenous EJCs and their RNA footprints (Figure 1A), providing a snapshot of the mRNA landscape before translation occurs. This has enabled researchers to identify EJCs on fully processed mRNA that haven't been subjected to ribosome-dependent decay. 2. Identifying New Splicing Events
Ripit operated in a legally gray area. In the United States, the Digital Millennium Copyright Act (DMCA) prohibits circumvention of CSS, even for personal backups. However, Ripit’s developers argued they provided only the tool, not instructions for piracy. No major lawsuit was filed against The Little App Factory, likely due to the small scale of the operation. The EJC is deposited on mRNA during splicing