Adn-388 Online

| Technique | Instrument | Conditions | |-----------|------------|------------| | Surface Plasmon Resonance (SPR) | Biacore T200 | PD‑L1 immobilized (500 RU), 25 °C, 0.1 M HEPES pH 7.4 | | Isothermal Titration Calorimetry (ITC) | MicroCal PEAQ‑ITC | 20 °C, 10 µM PD‑1, 100‑µM ADN‑388 | | Differential Scanning Fluorimetry (DSF) | CFX96 (Bio‑Rad) | 0.5 µM PD‑L1, 10‑µM SYPRO‑Orange |

| Property | Value | |----------|-------| | Molecular weight | 456.5 Da | | LogP (XlogP3) | 3.1 | | Aqueous solubility (pH 7.4) | 45 µM | | Plasma protein binding (rat) | 92 % | | CYP3A4 IC 50 | > 50 µM | | hERG inhibition (IC 50 ) | 31 µM | adn-388