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Even a tiny drop of extra liquid can throw off the concentration of a standard.
The class understood the assignment. This wasn't just chemistry; it was cartography. They were mapping the unknown. glucose standard curve
"Standard one," Mira called out. "0.15 Absorbance." "Standard two," Leo read the screen. "0.29 Absorbance." Even a tiny drop of extra liquid can
Leo looked at the graph. He traced the Y-axis up to 0.34. He slid his finger horizontally until it hit their perfect straight line, then dropped it straight down to the X-axis. They were mapping the unknown
"Right there," Leo said. "It corresponds to approximately 4.5 micrograms per milliliter."
Mira double-checked with the equation. 0.34 = 0.075(x) + 0.01. She solved for X. X = 4.4.
: Start with a high-concentration glucose stock (e.g., 2 mg/mL or 1 mM). Serial Dilutions : Use the formula