Introduction

The market for therapeutic antibodies has dramatically expanded over the past decades since their first approval in 1986.

Antibodies, which are proteins, form their structure and exert their activity through a complex series of non-covalent bonds and may lose their activity due to various external stimuli.

Therefore, the evaluation of structural stability is extremely important in the development and formulation of candidate antibodies.

Thermodynamic stability of antibodies is generally evaluated by DSC (differential scanning calorimetry) and circular dichroism (CD) spectroscopy.

Circular Dichroism spectroscopy is an easy and rapid method for obtaining information on the secondary and tertiary structure of proteins in solution and can be used to directly evaluate the protein structural change caused by heat.

Recently, Micsonai et al. developed the BeStSel algorithm that can accurately estimate the secondary structure composition from the CD spectrum by taking into account the parallel-antiparallel orientation of the β-strands and the twist of the antiparallel β-sheets.

BeStSel has the following features:

High estimation accuracy for a wide range of proteins, including β-structure-rich-proteins such as antibodies

Providing eight types of secondary structure information

A capability to predict the protein fold following the CATH classification

An open web server

While many academic researchers use the BeStSel web server, researchers in biopharma who need to work in a GxP environment have not been able to benefit from BeStSel.

To make BeStSel accessible to biopharma, JASCO developed Spectra Manager™ Ver.2.5 CFR BeStSel as an add-in software for Spectra Manager™, a control and analysis platform for CD spectrometers, which is compatible with GxP.

Covid And Ear Pressure ((full)) -

Viral infections like COVID-19 can create fluid buildup behind the eardrum. This fluid can become a breeding ground for bacteria, leading to a secondary ear infection. This often causes increased pressure, sharp pain, and sometimes fever.

Ear pressure from COVID-19 often overlaps with other common symptoms. Look for: covid and ear pressure

The connection between COVID-19 and ear pressure primarily involves inflammation and congestion in the upper respiratory tract. Here’s how it happens: Viral infections like COVID-19 can create fluid buildup

While ear pressure is usually temporary, certain signs indicate a need for professional care: covid and ear pressure

This is for informational purposes only. For medical advice or diagnosis, consult a professional. AI responses may include mistakes. Learn more